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KMID : 0191119870020010065
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Journal of Korean Medical Science
1987 Volume.2 No. 1 p.65 ~ p.70
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Purification of heat-labile enterotoxin from an enterotoxin from an enterotoxigenic Escherichiacoli of human origin by monoclonal immunoaffinity chromatography
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Cho MJ
Chang WH/Choi MS/Kim IS/Kang JS/Park KH/Kim HK/Cha CY/Chung HK/Rhee KH
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Abstract
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Heat-labile enterotoxin (LT) was purified from an enterotoxigenic Escherichia coli 015H11
of human origin. The purification steps included French pressure cell disruption of the bacteria, salting-out, DEAE-Sephacel on chromatography. Application of this procedure resulted in a 95.1-fold purification of LT with a yield of 19.9% as determined by rabbit ileal loop assay.
The final LT preparation showed only one protein-staining band on polyacrylamide gel
electrophoresis, indicating that the purified LT was homogeneous.
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